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What is a palindromic sequence in genetics?
What is a palindromic sequence in genetics?
What is a palindromic sequence in genetics?
Recombinant Dna And Biotechnology [1]
Restriction enzymes recognize specific DNA sequences and cut them in a predictable manner.. restriction endonucleases) are part of the genetic engineering toolbox and make gene cloning possible
As they are endonucleases, they can cut foreign DNA from the inside and make it ineffective.. What makes restriction enzymes suitable for gene cloning is that each of them has a specific restriction site
Most restriction enzymes recognize palindromic sequences, meaning that both strands of DNA will have the same sequence when read 5′ to 3′. As soon as this recognition occurs, enzyme cuts the sugar-phosphate backbone from specific points, which are generally within the restriction site
Which of the following palindromic bases sequences in DNA, can be easily cut at about the middle by some particular restriction enzyme?A. 5’CACGTA3’ and 3’CTCAGT5’B. 5’CGTTCG3’ and 3’ATGGTA5’C. 5’GATA [2]
Which of the following palindromic bases sequences in DNA, can be easily cut at about the middle by some particular restriction enzyme?. Hint: Palindromic sequences in DNA are the bases that read the same on both strands either 5 to 3 or 3 to 5 directions
The restriction enzymes are called molecular scissors and are responsible for cutting DNA. restriction enzymes serve as chemical knives to cut genes into defined fragments
It is their natural defense mechanism against bacteriophage infection. Some restriction enzymes such as EcoRI cut the strand of DNA a little away from the center of the palindrome sites, but between the same two bases on the opposite stands
[Solved] In the following palindromic base sequences of DNA, which on [3]
In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme?. – Palindrome in DNA refers to a sequence of base pairs that reads the same on the two strands when the orientation of the reading is kept the same.
– Restriction endonuclease is an enzyme that cuts DNA at or near specific recognition nucleotide sequences known as restriction sites.. – Restriction enzymes cut the strand of DNA a little away from the center of the palindrome sites, but between the same two bases on the opposite strands.
– Out of the four options, option (3) is the only palindromic sequence.. – Recognition sequences in DNA differ for each restriction enzyme, producing differences in the length, sequence, and strand orientation (5′ end or the 3′ end) of a sticky end of an enzyme restriction.
SOLVED: Which of the following is a palindromic restriction site? 5′ AGGCCT 3′ 5′ ACGCTG 3′ 5′ CTGCTG 3′ 5′ CGTTGC 3′ 5′ CGTACG 3 [4]
Get 5 free video unlocks on our app with code GOMOBILE. Which of the following is a palindromic restriction site? 5′ AGGCCT 3′ 5′ ACGCTG 3′ 5′ CTGCTG 3′ 5′ CGTTGC 3′ 5′ CGTACG 3
Fill in the Blanks: The sequences listed here represent half of a palindromic restriction site each. What is the complete sequence of each restriction site? A
Replace the Ns to complete the sequence of each restriction site: (points)5′ – ACGNNN – 3’5′ – AGNN – 3’5′ – ATCNNN – 3’5′ – NNNATC – 3. Which of the following is a palindrome sequence that could be recognized by one of type II restriction enzymes?Select one:a
Which of the following palindromic bases sequences in DNA, can be easily cut at about the middle by some particular restriction enzyme?A. 5’CACGTA3’ and 3’CTCAGT5’B. 5’CGTTCG3’ and 3’ATGGTA5’C. 5’GATA [5]
Which of the following palindromic bases sequences in DNA, can be easily cut at about the middle by some particular restriction enzyme?. Hint: Palindromic sequences in DNA are the bases that read the same on both strands either 5 to 3 or 3 to 5 directions
The restriction enzymes are called molecular scissors and are responsible for cutting DNA. restriction enzymes serve as chemical knives to cut genes into defined fragments
It is their natural defense mechanism against bacteriophage infection. Some restriction enzymes such as EcoRI cut the strand of DNA a little away from the center of the palindrome sites, but between the same two bases on the opposite stands
Palindromic Sequences [6]
Restriction enzymes cut double-stranded DNA* at specific locations based the pattern of bases found at those locations. These enzymes predictably cut both strands because the sequences they recognize are palindromic
Palindromic sequences are similar to language palindromes, but follow a distinct set of rules. Any string of bases can be made into a palindromic sequence by following these rules.
As a set of paired sequences (one on each of the strands of a double strand of DNA), the palindromes recognized by restriction enzymes follow a slightly different set of rules. They are probably more properly referred to as palindromic sequences to distinguish them from language palindromes
Palindromic sequence [7]
A palindromic sequence is a nucleic acid sequence in a double-stranded DNA or RNA molecule whereby reading in a certain direction (e.g. 5′ to 3′) on one strand is identical to the sequence in the same direction (e.g
The meaning of palindrome in the context of genetics is slightly different from the definition used for words and sentences. Since a double helix is formed by two paired antiparallel strands of nucleotides that run in opposite directions, and the nucleotides always pair in the same way (adenine (A) with thymine (T) in DNA or uracil (U) in RNA; cytosine (C) with guanine (G)), a (single-stranded) nucleotide sequence is said to be a palindrome if it is equal to its reverse complement
TGGATCCA because reversing the order of the nucleotides in the complement gives the original sequence.. A palindromic nucleotide sequence is capable of forming a hairpin
Recombinant Dna And Biotechnology [8]
Restriction enzymes recognize specific DNA sequences and cut them in a predictable manner.. restriction endonucleases) are part of the genetic engineering toolbox and make gene cloning possible
As they are endonucleases, they can cut foreign DNA from the inside and make it ineffective.. What makes restriction enzymes suitable for gene cloning is that each of them has a specific restriction site
Most restriction enzymes recognize palindromic sequences, meaning that both strands of DNA will have the same sequence when read 5′ to 3′. As soon as this recognition occurs, enzyme cuts the sugar-phosphate backbone from specific points, which are generally within the restriction site
BtrI, a novel restriction endonuclease, recognises the non-palindromic sequence 5′-CACGTC(-3/-3)-3′ [9]
BtrI, a novel restriction endonuclease, recognises the non-palindromic sequence 5′-CACGTC(-3/-3)-3′. The recognition sequence and cleavage positions of a new restriction endonuclease BtrI isolated from Bacillus stearothermophilus SE-U62 have been determined
Type II restriction endonucleases (ENases) include a group of 53 prototypes that recognise non-palindromic DNA sequences (ENases with recognition sequences that are interrupted by more than one base pair are not considered). Cleavage positions have been determined for 45 of these (1)
There are only a few so-called type IIQ restriction enzyme prototypes that cut both DNA strands symmetrically within their non-palindromic recognition sequences (3).. Here we report a new member of this rare subgroup of type II ENases.
Problem 7 Restriction sites are palindromi… [FREE SOLUTION] [10]
Restriction sites are palindromic, that is, they read the same in the \(5^{\prime}\) to \(3^{\prime}\) direction on each strand of DNA. What is the advantage of having restriction sites organized this way?
You performed all the steps correctly and electrophoresced the resulting DNA fragments correctly, but when you looked at the sequencing gel, many of the bands were duplicated (in terms of length) in other lanes. 94% of Vaia users get better grades.Sign up for free
[Solved] In the following palindromic base sequences of DNA, which on [11]
In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme?. – Palindrome in DNA refers to a sequence of base pairs that reads the same on the two strands when the orientation of the reading is kept the same.
– Restriction endonuclease is an enzyme that cuts DNA at or near specific recognition nucleotide sequences known as restriction sites.. – Restriction enzymes cut the strand of DNA a little away from the center of the palindrome sites, but between the same two bases on the opposite strands.
– Out of the four options, option (3) is the only palindromic sequence.. – Recognition sequences in DNA differ for each restriction enzyme, producing differences in the length, sequence, and strand orientation (5′ end or the 3′ end) of a sticky end of an enzyme restriction.
9.10: Palindromes [12]
A palindrome is a sequence of letters and/or words, that reads the same forwards and backwards. Palindromes that occur on opposite strands of the same section of DNA helix
The graphic shows the palindromic sequences “seen” by five restriction enzymes (named in blue) commonly used in recombinant DNA work.. In these cases, two different segments of the double helix read the same but in opposite directions.
Transcription factors are often dimers of identical proteins homodimers so it is not surprising that each member of the pair needs to “see” the same DNA sequence in the same orientation.. – The DNA of many transposons is flanked by inverted repeats such as this one:
Do degenerate recognition sites need to be palindromic? [13]
HomeFAQsDo degenerate recognition sites need to be palindromic?. FAQ: Do degenerate recognition sites need to be palindromic?
However, some enzymes have degenerate sites, meaning that they contain one or more base pairs that are not specifically defined (i.e., BsrFI-v2 recognizes RCCGGY, where R= A or G and Y= C or T). For degenerate enzymes, any base represented by the single letter code may be present at either location in the recognition site for cleavage to occur
Why do most restriction enzyme cuts at palindromic sequence? [14]
Why do most restriction enzyme cuts at palindromic sequence?. Enzymes such as restriction enzymes have to recognize a very specific sequence in order to carry out its task
Luckily! because you don’t want a ‘pacman’ that cuts DNA at random places.. DNA is double stranded, so it has ‘two sides’ to which the enzyme can bind
This means that the enzyme recognizes the sequence no matter from which side the enzyme approaches the DNA.. A palindromic sequence also increases the chance that both strands of DNA are cut
Structural Biochemistry/Nucleic Acid/DNA/Palindromic Sequencing [15]
Structural Biochemistry/Nucleic Acid/DNA/Palindromic Sequencing. A palindromic sequence is a sequence made up of nucleic acids within double helix of DNA and/or RNA that is the same when read from 5’ to 3’ on one strand and 5’ to 3′ on the other, complementary, strand
The pairing of nucleotides within the DNA double-helix is complementary which consist of Adenine (A) pairing with either Thymine (T) in DNA or Uracil (U) in RNA, while Cytosine (C) pairs with Guanine (G). So if a sequence is palindromic, the nucleotide sequence of one strand would be the same as its reverse complementary strand
This is the sequence where the restriction endonuclease, BamHI, binds to and cleaves at a specific cleavage site. When the complementary strand is read backwards, the sequence is 5’-GGATCC-3’ which is identical to the first one, making it a palindromic sequence.
Sources
- https://jackwestin.com/resources/mcat-content/recombinant-dna-and-biotechnology/restriction-enzymes#:~:text=restriction%20sites%3A%20specific%20sequences%20of,read%20in%20the%20same%20direction
- https://www.vedantu.com/question-answer/which-of-the-following-palindromic-bases-class-12-biology-cbse-5fe9abe54fe4296cd6ef39b5#:~:text=5’GAATTC3’%20and%203’CTTAG5’%20is,an%20example%20of%20palindrome%20DNA.
- https://testbook.com/question-answer/in-the-following-palindromic-base-sequences-of-dna–62da04a68b28f199e7374bc8#:~:text=Option%20(3)%20is%20the%20correct,%E2%80%94%E2%80%94%20CTTAAG%20%E2%80%94%E2%80%94%205′
- https://www.numerade.com/ask/question/which-of-the-following-is-a-palindromic-restriction-site-5-aggcct-3-5-acgctg-3-5-ctgctg-3-5-cgttgc-3-5-cgtacg3-79275/
- https://www.vedantu.com/question-answer/which-of-the-following-palindromic-bases-class-12-biology-cbse-5fe9abe54fe4296cd6ef39b5
- https://scienceprimer.com/palindromic-sequences
- https://en.wikipedia.org/wiki/Palindromic_sequence
- https://jackwestin.com/resources/mcat-content/recombinant-dna-and-biotechnology/restriction-enzymes
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC102641/
- https://www.hellovaia.com/textbooks/biology/concepts-of-genetics-11-edition/chapter-20/problem-7-restriction-sites-are-palindromic-that-is-they-rea/
- https://testbook.com/question-answer/in-the-following-palindromic-base-sequences-of-dna–62da04a68b28f199e7374bc8
- https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/Biology_(Kimball)/09%3A_Regulation_of_Gene_Expression/9.10%3A_Palindromes
- https://international.neb.com/faqs/0001/01/01/do-degenerate-recognition-sites-need-to-be-palindromic
- https://socratic.org/questions/why-do-most-restriction-enzyme-cuts-at-palindromic-sequence
- https://en.wikibooks.org/wiki/Structural_Biochemistry/Nucleic_Acid/DNA/Palindromic_Sequencing
